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KMID : 0861020190340060139
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Korea Journal of Herbology
2019 Volume.34 No. 6 p.139 ~ p.146
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Effect of herbal mixture (RAA) on hair growth in C57BL/6 mice
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Kim Yoo-Jin
Lee Ye-Ji
Kim Mi-Ryeo
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Abstract
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Objective : Recently, hair has become one of the important aspects of beauty. So, there are many studies about prevention and treatment of alopecia. Finasteride and minoxidil have been known to drug for alopecia treatment. However, these agents have side effects such as irritation, pruritus, and erythema when they were used for a long time. So, we assessed hair growth effect on herbal mixture (RAA) include in Rehmanniae Radix Preparata, Achyranthis Radix, and Acanthopanacis Cortex.
Methods : 6 weeks aged C57BL/6 mice were divided three treatment groups. : CON group (vehicle solution), MNXD group (positive control, 3% minoxidil), RAA group (15% RAA solution). And we applied 200 ㎕ of three groups to shaved dorsal skin every day. Hair growth effects of treatment were determined through phototrichogram by folliscope and hair follicle morphometry by H&E staining. And we assessed hair growth-related gene (VEGF, IGF-1, TGF-β1) expressions by western blot and 5-α reductase Ⅱ analysis of dermal (skin) or internal organ (prostate gland).
Results : Hair density and hair follicle size in MNXD or RAA group was enhanced compared to those of CON group, respectively. Also, the protein expression levels in dermal of VEGF, IGF-1 increased but TGF-β1 decreased in RAA and MNXD group, compared to CON group, respectively. 5-α reductase Ⅱ levels of tissues in MNXD or RAA group significantly decreased compared to those of CON group, respectively.
Conclusion : These results suggest that RAA has the promoting effect on hair growth.
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KEYWORD
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Herbal mixture, Hair growth, Alopecia, Minoxidil, C57BL/6 mice
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